A detailed linkage map of rainbow trout produced using doubled haploids

William P. Young, Paul A. Wheeler, Virginia H. Coryell, Paul S Keim, Gary H. Thorgaard

Research output: Contribution to journalArticle

259 Citations (Scopus)

Abstract

We report the first detailed genetic linkage map of rainbow trout (Oncorhynchus mykiss). The segregation analysis was performed using 76 doubled haploid rainbow trout produced by androgenesis from a hybrid between the 'OSU' and 'Arlee' androgenetically derived homozygous lines. Four hundred and seventy-six markers segregated into 31 major linkage groups and 11 small groups (<5 markers/group). The minimum genome size is estimated to be 2627.5 cM in length. The sex-determining locus segregated to a distal position on one of the linkage groups. We analyzed the chromosomal distribution of three classes of markers: (1) amplified fragment length polymorphisms, (2) variable number of tandem repeats, and (3) markers obtained using probes homologous to the 5' or 3' end of salmonid-specific small interspersed nuclear elements. Many of the first class of markers were clustered in regions that appear to correspond to centromeres. The second class of markers were more telomeric in distribution, and the third class were intermediate. Tetrasomic inheritance, apparently related to the tetraploid ancestry of salmonid fishes, was detected at one simple sequence repeat locus and suggested by the presence of one extremely large linkage group that appeared to consist of two smaller groups linked at their tips. The double haploid rainbow trout lines and linkage map present a foundation for further genomic studies.

Original languageEnglish (US)
Pages (from-to)839-850
Number of pages12
JournalGenetics
Volume148
Issue number2
StatePublished - 1998

Fingerprint

Oncorhynchus mykiss
Haploidy
Genome Size
Minisatellite Repeats
Genetic Linkage
Tetraploidy
Centromere
Microsatellite Repeats
Fishes

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Young, W. P., Wheeler, P. A., Coryell, V. H., Keim, P. S., & Thorgaard, G. H. (1998). A detailed linkage map of rainbow trout produced using doubled haploids. Genetics, 148(2), 839-850.

A detailed linkage map of rainbow trout produced using doubled haploids. / Young, William P.; Wheeler, Paul A.; Coryell, Virginia H.; Keim, Paul S; Thorgaard, Gary H.

In: Genetics, Vol. 148, No. 2, 1998, p. 839-850.

Research output: Contribution to journalArticle

Young, WP, Wheeler, PA, Coryell, VH, Keim, PS & Thorgaard, GH 1998, 'A detailed linkage map of rainbow trout produced using doubled haploids', Genetics, vol. 148, no. 2, pp. 839-850.
Young WP, Wheeler PA, Coryell VH, Keim PS, Thorgaard GH. A detailed linkage map of rainbow trout produced using doubled haploids. Genetics. 1998;148(2):839-850.
Young, William P. ; Wheeler, Paul A. ; Coryell, Virginia H. ; Keim, Paul S ; Thorgaard, Gary H. / A detailed linkage map of rainbow trout produced using doubled haploids. In: Genetics. 1998 ; Vol. 148, No. 2. pp. 839-850.
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