A common environmental contaminant affects sexual behavior in the clawed frog, Xenopus tropicalis

Angela L. Schwendiman, Catherine R Propper

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Behavior can be a sensitive measure of endocrine disruption from exposure to environmental contaminants. Xenopus tropicalis has become a developmental model system for evaluation of endocrine disrupting compounds because of its relatively rapid development and its sequenced, diploid genome. We used X. tropicalis as a model for endocrine disruption of sexual behavior. We injected frogs intraperitoneally (IP) with a gonadotropin hormone-releasing hormone agonist at 0.31μg/50μL or vehicle control solution and determined behavioral outcomes. Next, we used GnRH-induced sexual behavior to determine the effects of a 30-day exposure to aqueous estradiol (E2) at 10 -8M or a common pollutant, 4-tert octylphenol (OP), at the environmentally relevant concentrations of 10 -7M OP or 10 -8M OP. The GnRH-agonist treatment had no effect on female behaviors. In males, GnRH-agonist treatment increased approaches, touches, amplexus, and a sum total of all sexual behaviors (total sexual behavior score). Exposure to E2 or any dose of OP had no effect on female behaviors. In males, E2 and 10 -7M OP increased incidence of arm waving (a potential pheromone releasing behavior), and E2, and both doses of OP increased calling behavior compared to an unexposed control group. More males in all the exposure groups expressed sexual behavior than in the control group. This study demonstrates that a common pollutant, OP, affects male sexual behavior possibly by acting like an estrogen.

Original languageEnglish (US)
Pages (from-to)520-526
Number of pages7
JournalPhysiology and Behavior
Volume106
Issue number4
DOIs
StatePublished - Jun 25 2012

Fingerprint

Xenopus
Sexual Behavior
Anura
Gonadotropin-Releasing Hormone
Control Groups
Endocrine System
Behavior Control
Pheromones
Environmental Exposure
Touch
Diploidy
octylphenol
Frog
Estradiol
Estrogens
Genome
Hormones
Incidence
Therapeutics

Keywords

  • Amphibian
  • Endocrine disruption
  • Estrogen
  • Gonadotropin-releasing hormone
  • Mating behavior
  • Octylphenol
  • Sexual behavior
  • Xenopus tropicalis

ASJC Scopus subject areas

  • Behavioral Neuroscience
  • Experimental and Cognitive Psychology
  • Philosophy

Cite this

A common environmental contaminant affects sexual behavior in the clawed frog, Xenopus tropicalis. / Schwendiman, Angela L.; Propper, Catherine R.

In: Physiology and Behavior, Vol. 106, No. 4, 25.06.2012, p. 520-526.

Research output: Contribution to journalArticle

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